Combining different mutations into the same variant often does not result in an additive effect, instead the mutations can antagonise or synergise, a phenomenon called epistasis.

This tool calculates the epistatic effect different mutations can have on a protein structure.

For example, if a mutant has 3 mutations, there are different combinations possible: Three single mutants and three double mutants. The creation of these 6 mutants as well as the parent protein and triple mutant make a total of 8 mutants. This would result in a complete dataset (full deconvolution of a triple mutant). Alternatively, it is not necessary to deconvolute all mutations from a multi-mutational variant, since this would be very time consuming. Instead, it is possible to include different subsets of mutations in double, triple, quadruple or nth mutants.

In either case, this site accepts two ways of inputting the data. The default is by generating and filling out a table online, the second is by generating and filling out an Excel spreadsheet and uploading it. The data can be in various replicates. Cases with a single replicated are accepted. Leave a replicate blank if missing.

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mutation N
replicate N

mutation number
replicate number